منابع مشابه
Agarose drop method for loading thin polyacrylamide gels.
Thin gels (<I mm) can be loaded conveniently, rapidly, and quantitatively by suspending the sample to be analyzed in a drop of agarose gel and simply placing the solidified drop on top of the stacking gel. With this method there is no lower limit to the size of the sample to be loaded or to the thinness of the gel to be employed. Using the silver stain quantitation of between 1 and 1000 @ample ...
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b-cyclodextrin glucosyl transferase (b-CGTase) hydrolyses starch to produce b-cyclodextrin by transglycosylation (cyclization) activity. The conventional method for detection of b-CGTase activity is based on detecting starch hydrolysis by iodine staining. This method reveals all amylolytic enzymes, but can not discriminate them. In the present study, we introduce a new method for specific detec...
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We have developed a simple, rapid method to isolate intact radioactively labelled RNA (either with ^ P or S) from dried polyacrylamide gels on Whatman paper, thus allowing its further investigation even after several months (in the case of a Slabel) of handling the gels without any protection, e.g. from RNases. The isolated RNA molecules are still biologically active, since we could demonstrate...
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A sensitive method is described for the detection of glycoproteins after polyacrylamide gel electrophoresis. Dansyl hydrazine is condensed with aldehydic functions generated by periodate oxidation of protein bound carbohydrate. The resulting hydrazones are reduced to the stable hydrazine derivatives with sodium borohydride. After destaining, the fluorescent-labeled glycoproteins are visualized ...
متن کاملA fast and inexpensive procedure for drying polyacrylamide gels.
spective gel image revealed that the density of the area between 200 and 1600 bp was very similar in all of the four lanes. Therefore, volume-corrected calculation of this experiment (Fig. 1B, filled columns) displayed results comparable to the densitometric analysis of Fig. 1A (shown in Fig. 1B, open columns). Thus, this method offers reproducible results as a function of cell number but indep...
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ژورنال
عنوان ژورنال: Protocol Exchange
سال: 2011
ISSN: 2043-0116
DOI: 10.1038/protex.2011.222